Last updated: March 2, 2026 by Maria Gonzalez

Understanding the Dilution Equation C1V1 = C2V2

Formula

The dilution equation C1V1 = C2V2 is one of the most frequently used formulas in chemistry and biology laboratories. It relates the concentration and volume of a stock solution to the concentration and volume of the diluted solution.

This equation works because the number of moles of solute remains constant during dilution — only solvent is added. By rearranging the equation, you can solve for any unknown variable when the other three are known.

Common use cases:

  • Laboratory solution preparation
  • Pharmaceutical compounding
  • Serial dilution series

Frequently Asked Questions

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Maria Gonzalez

Registered Dietitian, RD, MPH

Maria is a Registered Dietitian with a Master's in Public Health. She focuses on evidence-based nutrition assessment tools including BMI, calorie calculations, and body composition analysis.

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